UBE2T/FANCL-IN-1 is primarily utilized in research focused on understanding and targeting the Fanconi anemia (FA) DNA repair pathway. This compound acts as an inhibitor of the UBE2T-FANCL interaction, which plays a critical role in the ubiquitination process essential for DNA damage repair. By disrupting this interaction, researchers can study the mechanisms underlying FA and explore potential therapeutic strategies for cancers associated with defects in the FA pathway. Its application is particularly relevant in preclinical studies aimed at developing targeted therapies for tumors with compromised DNA repair mechanisms. Additionally, it serves as a valuable tool in biochemical assays to dissect the molecular intricacies of ubiquitin-mediated signaling in cellular stress responses.