iGOT1-01 is primarily used in biochemical research to study and modulate the function of the mitochondrial glutamate-oxaloacetate transaminase 1 (GOT1) enzyme. This compound is particularly valuable in cancer research, where it helps investigate metabolic reprogramming in cancer cells, especially in contexts like pancreatic ductal adenocarcinoma. By inhibiting GOT1, researchers can explore its role in altering cellular metabolism, particularly the shift between glycolysis and the tricarboxylic acid (TCA) cycle. This provides insights into potential therapeutic strategies targeting cancer cell metabolism. Additionally, iGOT1-01 is utilized in studies focused on understanding cellular responses to oxidative stress and nutrient deprivation, contributing to broader knowledge in metabolic diseases and cellular physiology.