HLI373 is primarily utilized in research and development within the field of biochemistry and molecular biology. It serves as a potent and selective inhibitor of histone deacetylase (HDAC) enzymes, which are involved in regulating gene expression and cellular processes such as cancer development. Its application is significant in studying the mechanisms of HDAC activity and epigenetic modifications in diseases like cancer and neurological disorders. Researchers use it to explore potential therapeutic strategies by inhibiting HDAC, which can help in understanding disease progression, identifying new drug targets, and developing more effective treatments. Additionally, it is employed in laboratory settings to investigate the effects of HDAC inhibition on cell growth, survival, gene expression changes, and other cellular processes, contributing to advancements in biomedical science.