SMI-16a is primarily utilized in research settings to study its effects on cellular pathways, particularly those involving cell cycle regulation and apoptosis. It is often employed in laboratory experiments to investigate its potential as an inhibitor of specific proteins or enzymes that play a role in cancer progression. Researchers use SMI-16a to explore its ability to disrupt tumor growth or enhance the efficacy of other therapeutic agents. Additionally, it serves as a valuable tool in understanding molecular mechanisms underlying diseases, aiding in the development of targeted therapies. Its application is largely confined to preclinical studies, where it helps identify promising leads for further drug development.