Sp-cAMPS is widely used in biochemical and pharmacological research as a potent and selective activator of protein kinase A (PKA). It mimics the action of cyclic AMP (cAMP) by binding to the regulatory subunits of PKA, leading to their dissociation and activation of the catalytic subunits. This property makes it a valuable tool for studying cAMP-dependent signaling pathways in cells and tissues. Researchers utilize Sp-cAMPS to investigate the role of PKA in various physiological processes, including metabolism, gene expression, and cellular differentiation. Additionally, it is employed in studies focusing on the effects of PKA activation on neuronal function, cardiac contractility, and hormone secretion. Its ability to selectively activate PKA without affecting other cAMP-dependent proteins makes it a precise reagent for dissecting complex signaling networks.