Cholera toxin is widely used in scientific research to study cellular processes, particularly those involving G proteins and signal transduction pathways. It is a valuable tool for investigating the mechanism of action of G protein-coupled receptors (GPCRs) and their role in various physiological functions. By ADP-ribosylating the Gs alpha subunit, it permanently activates adenylate cyclase, leading to increased cyclic AMP (cAMP) levels. This property makes it useful for studying cAMP-mediated cellular responses, such as ion transport, secretion, and gene expression. Additionally, cholera toxin is employed in immunology to enhance immune responses, often serving as an adjuvant in vaccine development to improve antigen presentation and stimulate stronger immune reactions. Its ability to modulate signaling pathways also contributes to its use in cancer research, where it helps explore the role of cAMP in cell proliferation and differentiation.