Rhod-2 AM is widely used in biological research as a calcium indicator. It is particularly effective in studying intracellular calcium dynamics in live cells. The compound is membrane-permeable, allowing it to easily enter cells. Once inside, it is hydrolyzed by intracellular esterases, trapping the fluorescent dye within the cell. Upon binding to calcium ions, Rhod-2 exhibits a significant increase in fluorescence, enabling researchers to monitor calcium fluctuations in real-time. This makes it a valuable tool in neuroscience, cardiology, and cell biology for understanding calcium signaling pathways, cellular responses to stimuli, and mechanisms of diseases related to calcium dysregulation. Its high sensitivity and selectivity for calcium make it a preferred choice for imaging experiments in live tissues and cells.