Used primarily in biological and medical research, this buffer solution is essential for maintaining stable pH levels during experiments involving enzymes, proteins, and cellular components. It is particularly useful in electrophoresis techniques, where it helps in the separation of nucleic acids and proteins by providing a consistent ionic environment. The inclusion of EDTA in the buffer is crucial for chelating metal ions, which prevents enzymatic degradation and ensures the integrity of biological samples. Additionally, this buffer is often employed in immunoassays and histochemical staining procedures, where precise pH control is necessary for accurate results. Its gelatin component aids in stabilizing proteins and preventing nonspecific binding, making it a reliable choice for sensitive biochemical applications.