Tris HCl is widely used as a buffering agent in biochemical and molecular biology applications. It helps maintain a stable pH in various experimental setups, such as protein electrophoresis, nucleic acid purification, and enzyme assays. Its effectiveness in the pH range of 7.0 to 9.0 makes it suitable for many biological systems. In cell culture, Tris HCl is often employed to stabilize the pH of growth media, ensuring optimal conditions for cell proliferation. It is also a key component in DNA and RNA extraction protocols, where it aids in maintaining the integrity of nucleic acids during isolation. Additionally, Tris HCl is used in diagnostic kits and reagents, particularly in immunoassays and clinical chemistry, to ensure accurate and consistent results. Its buffering capacity and compatibility with biological molecules make it a versatile choice in research and industrial laboratories.