Tris Buffered Saline (TBS) is widely used in biological and biochemical research as a reliable buffer solution. It is commonly employed for washing and diluting samples in immunoassays, such as Western blotting and ELISA, due to its ability to maintain a stable pH and provide optimal conditions for antibody-antigen interactions. TBS is also utilized in immunohistochemistry for tissue section rinsing, ensuring minimal background staining and preserving cellular structures. Additionally, it serves as a base for preparing blocking solutions to prevent non-specific binding in various assays. Its compatibility with biological systems makes it a preferred choice for cell culture applications, including cell washing and media preparation. TBS is also used in molecular biology techniques, such as DNA and RNA hybridization, to maintain consistent ionic strength and pH. Its versatility and effectiveness in maintaining physiological conditions make it an essential reagent in laboratories.