Tris Buffered Saline with Tween 20 is widely used in biological and biochemical applications due to its ability to maintain stable pH and reduce non-specific binding. It is commonly employed as a washing buffer in immunoassays, such as ELISA and Western blotting, to remove unbound antibodies or proteins while minimizing background noise. The inclusion of Tween 20, a non-ionic detergent, helps to disrupt hydrophobic interactions and prevent proteins from sticking to surfaces or membranes. This makes it particularly useful for blocking and washing steps in protein and antibody-based experiments. Additionally, it is utilized in cell culture and molecular biology techniques to maintain cell viability and ensure consistent experimental conditions. Its versatility and effectiveness in stabilizing biological samples make it a staple in laboratories focused on protein analysis and diagnostics.