Tricine buffered saline is widely used in biochemical and molecular biology research due to its effective buffering capacity in the pH range of 7.4 to 8.0. It is commonly employed in protein and nucleic acid electrophoresis, particularly for separating low molecular weight proteins in polyacrylamide gels. The buffer is also utilized in enzyme assays and immunoblotting techniques, where maintaining a stable pH is critical for optimal activity and binding. Additionally, tricine buffered saline is suitable for cell culture applications, as it provides a physiological environment for cell maintenance and experimentation. Its compatibility with various biological systems makes it a versatile choice in laboratory protocols.