Tricine buffer is widely used in biochemical and molecular biology applications due to its effective buffering capacity in the pH range of 7.4 to 8.8. It is particularly useful in protein and peptide electrophoresis, especially for separating low molecular weight proteins (below 100 kDa) in SDS-PAGE. Tricine buffer helps maintain stable pH conditions, ensuring accurate and reproducible results. Additionally, it is employed in enzyme assays, cell culture media, and other biochemical studies where a mild buffering system is required. Its low ionic strength and minimal interference with biological processes make it a preferred choice in various laboratory protocols.