Tricine buffer is widely used in biochemical and molecular biology research due to its effective buffering range in the slightly alkaline pH region, typically between pH 7.4 and 8.8. It is particularly favored in electrophoresis techniques, such as SDS-PAGE, for separating small proteins and peptides, as it provides better resolution compared to other buffers. Additionally, Tricine is employed in enzyme assays and protein purification processes where maintaining a stable pH is critical. Its low ionic strength and minimal interference with biochemical reactions make it suitable for use in cell culture media and other biological applications. Tricine buffer is also utilized in the study of photosynthesis and chloroplast isolation due to its compatibility with biological membranes and minimal impact on photosynthetic activity.