Tricine buffer is widely used in biochemical and molecular biology research due to its effective buffering capacity in the pH range of 7.4 to 8.8. It is particularly favored in protein and peptide studies, as it minimizes interference with low molecular weight proteins during electrophoresis. Tricine is commonly employed in SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) for separating small proteins and peptides, providing better resolution compared to traditional glycine-based buffers. Additionally, it is used in enzyme assays, cell culture media, and as a stabilizing agent in various biochemical reactions. Its low UV absorbance makes it suitable for applications involving spectrophotometric analysis. Tricine buffer is also utilized in plant tissue culture and as a component in formulations for studying oxidative stress and antioxidant activity. Its versatility and compatibility with biological systems make it a valuable tool in laboratory research.