Tricine buffer is widely used in biochemical and molecular biology applications due to its effective buffering capacity in the pH range of 7.4 to 8.8. It is particularly useful in protein electrophoresis, especially for separating low molecular weight proteins and peptides, as it minimizes glycine interference. Tricine is also employed in enzyme assays and cell culture media, where it helps maintain stable pH conditions. Additionally, it is utilized in the preparation of samples for mass spectrometry and in the study of membrane proteins, where its compatibility with detergents is advantageous. Its low ionic strength makes it suitable for applications requiring minimal interference with biological processes.