1X Tris-Glycine buffer is widely used in electrophoresis, particularly in SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis), for separating proteins based on their molecular weight. It provides the optimal pH and ionic conditions for the migration of proteins through the gel matrix, ensuring clear and precise band resolution.

Additionally, it is employed in Western blotting as a transfer buffer to move proteins from the gel to a membrane for further analysis. The buffer’s compatibility with various staining and detection methods makes it a versatile choice in protein research and diagnostics. Its stability and effectiveness in maintaining consistent pH levels during experiments contribute to reliable and reproducible results.