Used primarily in molecular biology and biochemistry for preparing Tris-buffered saline (TBS) solutions, it is essential for maintaining stable pH levels during experiments. It is commonly employed in Western blotting for washing membranes and diluting antibodies, ensuring optimal binding conditions. The buffer is also utilized in immunohistochemistry to rinse tissue sections, reducing background staining. Additionally, it supports cell culture applications by providing a physiological environment for cell maintenance and experimentation. Its ready-to-use premix format simplifies preparation, saving time and ensuring consistency in laboratory workflows.