TBE buffer is widely used in molecular biology for agarose gel electrophoresis, a technique that separates DNA fragments by size. It provides the necessary ionic environment for the movement of DNA through the gel matrix. The buffer's components help maintain a stable pH and conductivity, ensuring consistent and accurate results. TBE buffer is particularly suitable for resolving smaller DNA fragments, making it a preferred choice for applications like PCR product analysis, restriction enzyme digestion, and DNA sequencing. Additionally, it is used in nucleic acid staining and visualization processes, where it supports the binding of dyes like ethidium bromide to DNA for detection under UV light.