SSPE Buffer (PBS-EDTA) is widely used in molecular biology and biochemistry for various applications. It is particularly effective in nucleic acid hybridization techniques, such as Southern and Northern blotting, where it helps maintain the stability and integrity of DNA and RNA during the hybridization process. The buffer's combination of phosphate-buffered saline (PBS) and ethylenediaminetetraacetic acid (EDTA) ensures optimal pH and chelates divalent cations, preventing nuclease activity that could degrade nucleic acids. Additionally, SSPE Buffer is utilized in washing steps to remove non-specific binding and reduce background noise in hybridization experiments. Its compatibility with membrane-based assays makes it a crucial component in ensuring accurate and reliable results in genetic research and diagnostics.