PBS buffer premixed powder, pH 7.4, is widely used in biological and chemical laboratories for various applications. It is commonly employed as a diluent or washing solution in cell culture and molecular biology experiments. The buffer is ideal for maintaining a stable pH environment during procedures such as immunoassays, ELISA, and Western blotting. It is also used in the preparation of samples for flow cytometry and in the isolation and purification of proteins and nucleic acids. Additionally, PBS buffer is utilized in immunohistochemistry to rinse tissues and cells, ensuring minimal disruption to their natural state. Its isotonic and non-toxic properties make it suitable for use in vivo and in vitro studies, including cell viability assays and drug delivery research. The premixed powder form offers convenience and consistency, reducing preparation time and ensuring reliable results.