MOPS buffer is widely used in biological and biochemical research due to its effective buffering capacity in the pH range of 6.5 to 7.9. It is commonly employed in electrophoresis, particularly for RNA separations, as it provides a stable pH environment that minimizes RNA degradation. MOPS is also utilized in cell culture applications, where it helps maintain optimal pH conditions for cell growth and viability. Additionally, it is a preferred buffer in protein purification processes and enzyme assays, ensuring consistent and reliable results. Its low reactivity with metal ions makes it suitable for use in studies involving metal-dependent enzymes or proteins. Overall, MOPS buffer is a versatile tool in maintaining pH stability across various experimental conditions.