MOPS buffer is widely used in biochemical and molecular biology research due to its effective buffering capacity in the pH range of 6.5 to 7.9. It is commonly employed in electrophoresis, particularly for RNA agarose gel electrophoresis, as it provides stable pH conditions and minimizes RNA degradation. MOPS is also utilized in cell culture media to maintain a consistent pH environment, ensuring optimal cell growth and viability. Additionally, it is applied in protein purification and chromatography processes to stabilize proteins and maintain their functionality. Its compatibility with various biochemical assays and its ability to resist metal ion interference make it a versatile choice in laboratory applications.