MOPS buffer is widely used in biological and biochemical research due to its ability to maintain stable pH conditions. It is commonly employed in electrophoresis, particularly for RNA and DNA separation, as it provides consistent buffering capacity without interfering with nucleic acids. MOPS is also utilized in cell culture media to maintain physiological pH levels, ensuring optimal growth conditions for cells. Additionally, it is applied in protein purification processes, where it helps stabilize proteins and maintain their functionality. Its low UV absorbance makes it suitable for spectroscopic analyses, ensuring accurate measurements without interference. MOPS buffer is also used in enzyme assays, where it helps maintain the required pH for enzyme activity and stability.