Alkaline Phosphatase Buffer-2 is widely used in molecular biology and biochemistry laboratories for enzymatic reactions involving alkaline phosphatase. It is essential in processes such as dephosphorylation of DNA, RNA, and proteins, which is a critical step in cloning experiments to prevent self-ligation of vectors. The buffer provides optimal pH and ionic conditions for the enzyme to function efficiently, ensuring high activity and stability during reactions. Additionally, it is employed in ELISA and other immunoassay techniques for signal detection, where alkaline phosphatase is used as a reporter enzyme. Its consistent performance makes it a reliable choice for researchers aiming to achieve accurate and reproducible results in various experimental setups.