Alkaline Phosphatase Buffer-2 is widely used in molecular biology and biochemistry laboratories for enzymatic assays, particularly those involving alkaline phosphatase. It provides an optimal pH environment for the enzyme to function efficiently, ensuring accurate and reliable results in experiments such as ELISA, Western blotting, and DNA/RNA hybridization techniques. The buffer is also essential in dephosphorylation reactions, where it aids in removing phosphate groups from nucleic acids or proteins, a critical step in cloning and sequencing workflows. Additionally, it is employed in histochemistry for staining tissues to detect alkaline phosphatase activity, helping in the study of cellular processes and disease mechanisms. Its stability and consistency make it a preferred choice for researchers requiring precise enzymatic activity control.