Alkaline Phosphatase Buffer-2 is widely used in molecular biology and biochemistry laboratories for enzymatic reactions involving alkaline phosphatase. It is essential in dephosphorylation processes, such as preparing DNA or RNA for cloning by removing 5' phosphate groups to prevent self-ligation. This buffer is also employed in ELISA and Western blotting techniques to enhance the detection of target proteins or nucleic acids. Its optimized pH and composition ensure maximum enzyme activity, making it a reliable choice for research and diagnostic applications.