Used as a buffering agent in biochemical and cellular studies due to its excellent pH stability in physiological ranges. It maintains consistent pH in enzyme-catalyzed reactions and cell culture systems. Commonly applied in protein purification, chromatography, and electrophoresis where pH control is critical. Its zwitterionic nature minimizes interference with biological processes, making it suitable for use in sensitive assays and diagnostic applications. Also utilized in some pharmaceutical formulations to stabilize active ingredients.