Used in biochemical and molecular biology laboratories to maintain a stable pH environment during experiments, especially in procedures involving enzymes or proteins that are sensitive to pH changes. Commonly applied in DNA and RNA extraction protocols, electrophoresis, and chromatography to ensure optimal activity and stability of biomolecules. Also utilized in buffer systems for cell lysis and protein purification due to its compatibility with biological samples. The buffer effectively resists pH changes in the range near neutrality, making it suitable for physiological pH applications.