POPSO hydrate is commonly used as a buffering agent in biochemical and biological research. It is particularly effective in maintaining stable pH levels in cell culture media and enzyme assays, ensuring optimal conditions for cellular growth and enzymatic activity. This compound is also utilized in protein purification processes, where it helps to stabilize proteins and prevent denaturation. Additionally, POPSO hydrate is employed in electrophoresis and chromatography techniques, providing consistent pH environments crucial for accurate separation and analysis of biomolecules. Its buffering capacity in the physiological pH range makes it a valuable tool in various laboratory applications requiring precise pH control.