Used primarily in molecular biology and biochemistry, RNase-Free Phosphate Buffered Saline is essential for experiments involving RNA to prevent degradation. It is commonly employed in RNA extraction, purification, and handling processes, ensuring samples remain intact and uncontaminated. The solution is also utilized in cell culture applications, where it serves as a washing buffer to maintain cell viability and remove debris. Additionally, it is a key component in immunoassays, such as ELISA, for diluting antibodies or washing steps to minimize background noise. Its RNase-free property makes it critical for sensitive RNA-related research, including PCR, qPCR, and RNA sequencing workflows.