Sucrose in MNE buffer is widely used in biological research for cell fractionation and isolation of cellular organelles. It helps maintain osmotic balance, preventing cell lysis during centrifugation. This solution is particularly useful in separating mitochondria, nuclei, and other organelles by density gradient centrifugation. Additionally, it stabilizes membrane structures, preserving their integrity for further analysis. Sucrose in MNE buffer is also employed in cryopreservation to protect cells and tissues from ice crystal damage during freezing. Its applications extend to protein crystallization studies, where it aids in maintaining protein stability and promoting crystal formation.