Phosphate Buffer (PB Buffer) is widely used in biological and chemical research to maintain a stable pH environment. It is essential in cell culture applications, where it helps to keep the pH within a narrow range, ensuring optimal conditions for cell growth and function. In molecular biology, PB Buffer is frequently employed in techniques such as Western blotting, ELISA, and immunohistochemistry, where it aids in maintaining the stability of proteins and antibodies during the assay process. In biochemistry, PB Buffer is used to prepare samples for electrophoresis, ensuring that proteins and nucleic acids remain stable and properly separated. It is also utilized in enzyme activity assays, where a consistent pH is crucial for accurate measurement of enzymatic reactions. Additionally, PB Buffer is commonly used in the preparation of reagents and solutions for various laboratory procedures, providing a reliable and consistent pH environment. In medical and clinical laboratories, PB Buffer is used in diagnostic tests and procedures, including blood analysis and tissue staining, where it helps to maintain the integrity of biological samples. Its ability to resist changes in pH makes it an ideal choice for applications requiring precise and stable conditions.