Phosphate Buffer is widely used in biological and chemical research to maintain a stable pH environment. It is essential in cell culture applications, ensuring that cells grow under optimal conditions. In molecular biology, it is used for DNA and RNA extractions, as well as in protein purification processes to stabilize proteins and prevent denaturation. The buffer is also employed in diagnostic assays, such as ELISA, to provide a consistent pH for accurate results. Additionally, it is used in pharmaceutical formulations to maintain the stability and efficacy of drugs. In biochemistry, it is crucial for enzyme activity studies, as enzymes often require a specific pH range to function properly. Phosphate Buffer is also utilized in electrophoresis techniques to separate and analyze biomolecules. Its versatility and reliability make it a fundamental component in various scientific and industrial applications.