Phosphate Buffer (PB Buffer), 0.2 M at pH 8.5, is widely used in biological and chemical research due to its ability to maintain a stable pH. It is commonly employed in cell culture applications to provide a consistent environment for cell growth and experiments. In molecular biology, PB Buffer is utilized for washing and resuspending cells, as well as in procedures like immunoassays and protein purification. It is also a key component in electrophoresis techniques, ensuring the stability of biomolecules during separation. Additionally, PB Buffer is used in diagnostic assays and as a diluent for antibodies and other reagents, ensuring accurate and reproducible results. Its compatibility with various biological systems makes it a versatile tool in laboratories.