Sodium Phosphate Buffer (PBS Buffer) is widely used in biological and biochemical research for maintaining stable pH levels in various experimental procedures. It is essential in cell culture applications, where it provides a balanced environment for cell growth and maintenance. PBS Buffer is also utilized in immunoassays, such as ELISA, to wash and dilute samples, ensuring minimal interference with antibody-antigen interactions. In molecular biology, it is employed for DNA and RNA extraction, as well as in protein purification processes. Additionally, PBS Buffer serves as a diluent for reagents and a rinsing solution for laboratory equipment, ensuring consistency and accuracy in experimental results. Its isotonic and non-toxic properties make it suitable for use in in vivo studies and medical applications, such as intravenous drug administration and tissue preservation.