Phosphate Buffered Saline (PBS) is widely used in biological and biochemical research as a buffer solution. It is essential for maintaining a stable pH in cell cultures, ensuring that cells remain in an environment similar to their natural conditions. PBS is commonly employed in washing cells during experiments to remove residual media, proteins, or other contaminants without causing osmotic stress. It is also used as a diluent for reagents and samples in various assays, including ELISA and immunohistochemistry, to maintain consistency and accuracy. In molecular biology, PBS serves as a medium for suspending and transporting biological samples. Additionally, it is utilized in medical and clinical settings for rinsing wounds, rehydrating patients, and as a base for intravenous drug formulations. Its compatibility with living tissues makes it a versatile and indispensable tool in both research and healthcare applications.