Succinate buffer is widely used in biochemical and molecular biology research due to its effective buffering capacity in the pH range of 4.5 to 6.0. It is commonly employed in enzyme assays, particularly for enzymes that function optimally in slightly acidic conditions, such as certain dehydrogenases and phosphatases. The buffer is also utilized in protein purification processes, where maintaining a stable pH is critical for preserving protein structure and activity. In cell culture applications, succinate buffer helps maintain the pH of the medium, ensuring optimal growth conditions for cells. Additionally, it is used in electrophoresis and chromatography techniques to stabilize the pH of the running buffer, improving the resolution and reproducibility of separations. Its compatibility with various biological systems makes it a versatile choice in laboratory settings.