Succinate buffer is widely used in biochemical and molecular biology research due to its effective pH stabilization properties, typically in the range of 4.5 to 6.0. It is commonly employed in enzyme assays, particularly for enzymes that function optimally in slightly acidic conditions, such as certain proteases and phosphatases. The buffer is also utilized in protein purification processes, where maintaining a stable pH is crucial for preserving protein structure and activity. Additionally, succinate buffer finds application in cell culture studies, where it helps maintain a consistent environment for cell growth and metabolic activity. Its compatibility with various biochemical reagents and its ability to resist pH changes make it a reliable choice in laboratory settings.