Succinate buffer is widely used in biochemical and molecular biology research due to its effective pH stabilization properties within the range of 4.5 to 6.0. It is commonly employed in enzyme assays, particularly for enzymes that function optimally in slightly acidic conditions, such as certain dehydrogenases and phosphatases. The buffer is also utilized in protein purification processes, where maintaining a consistent pH is crucial for preserving protein structure and activity. Additionally, succinate buffer finds application in cell culture studies, where it helps maintain the physiological pH of the medium, ensuring optimal cell growth and function. Its compatibility with various biochemical reagents and stability under a range of experimental conditions makes it a reliable choice for numerous laboratory applications.