Used extensively in biochemical research to study kinase activity, this buffer is essential for maintaining optimal pH and ionic conditions during enzymatic assays. It ensures the stability and functionality of kinases, facilitating accurate measurement of phosphorylation events. Commonly employed in drug discovery and development, it helps identify potential kinase inhibitors by providing a consistent environment for high-throughput screening. Additionally, it supports protein-protein interaction studies and signal transduction pathway analysis, making it a critical tool in understanding cellular processes and disease mechanisms.