Used primarily in biochemical research, it facilitates the study of kinase enzymes, which are crucial for understanding cellular signaling pathways. The buffer provides optimal pH and ionic conditions, ensuring stable enzyme activity during assays. It is commonly employed in phosphorylation studies, helping to identify substrates and inhibitors of kinases. This is essential for drug discovery, particularly in developing treatments for diseases like cancer, where kinase activity is often dysregulated. Additionally, it supports high-throughput screening processes, enabling researchers to efficiently test large libraries of compounds for potential therapeutic effects.