Mcilvaine Buffer is widely used in various biochemical and microbiological applications due to its ability to maintain a stable pH. It is particularly useful in enzyme assays, where precise pH control is critical for accurate results. In microbiology, it is employed to prepare media and solutions for culturing microorganisms, ensuring optimal growth conditions. Additionally, Mcilvaine Buffer is utilized in chromatography and electrophoresis techniques to maintain the pH of the mobile phase or running buffer, which is essential for the separation and analysis of biomolecules. Its buffering capacity also makes it suitable for use in diagnostic kits and research studies involving protein stability and solubility.