Mcilvaine Buffer is widely used in biochemical and microbiological research for maintaining stable pH levels in various experimental conditions. It is particularly valuable in enzyme assays, where precise pH control is critical for accurate results. The buffer is also employed in the preparation of samples for electrophoresis, ensuring consistent pH during protein and nucleic acid separation. In microbiology, Mcilvaine Buffer is utilized for culturing and studying microorganisms, as it provides a stable environment for growth. Additionally, it is used in diagnostic procedures, such as immunoassays, to maintain optimal pH for antigen-antibody interactions. Its versatility and reliability make it a preferred choice in laboratories for a wide range of applications.