Mcilvaine Buffer is widely used in various biochemical and microbiological applications due to its ability to maintain a stable pH range. It is commonly employed in enzyme assays, where precise pH control is crucial for optimal enzyme activity and accurate results. In cell culture studies, it helps maintain the physiological pH of the medium, ensuring proper cell growth and function. Additionally, Mcilvaine Buffer is utilized in chromatography techniques, such as HPLC, to stabilize the pH of the mobile phase, improving separation efficiency and reproducibility. It is also used in diagnostic assays, including ELISA, to provide a consistent pH environment for antigen-antibody interactions. Furthermore, this buffer is applied in environmental testing to analyze water and soil samples, ensuring reliable pH conditions for accurate measurements. Its versatility makes it a valuable tool in research, diagnostics, and quality control processes.