Veronal Buffered Saline with Mg and EGTA (VBSMG) is primarily used in immunology and biochemistry research for maintaining the stability of biological samples. It is particularly effective in preserving the integrity of cell membranes and proteins during experiments. The inclusion of magnesium (Mg) supports enzymatic activities, especially those of magnesium-dependent enzymes, while EGTA chelates calcium ions, preventing unwanted calcium-mediated processes. This makes VBSMG ideal for studies involving complement system assays, cell lysis protocols, and other applications where precise control of divalent cations is critical. Its buffering capacity ensures a stable pH, essential for consistent experimental conditions.