Veronal Buffered Saline with EDTA (VBSE) is primarily used in immunology and serology for various laboratory applications. It serves as a diluent for antibodies and antigens, ensuring stability and optimal conditions for immunological reactions. VBSE is commonly employed in complement fixation tests, where it helps maintain the integrity of complement proteins. The EDTA component acts as a chelating agent, preventing metal ion interference, which is crucial for accurate results. Additionally, VBSE is utilized in flow cytometry for cell suspension preparation, as it preserves cell viability and prevents clumping. Its buffering capacity ensures a stable pH, making it suitable for sensitive biological assays.