Imidazole buffered saline is widely used in biochemical and molecular biology research due to its ability to maintain a stable pH environment. It is particularly effective in protein studies, where it helps in the purification and stabilization of proteins during electrophoresis and chromatography. The buffer is also utilized in immunoassays, such as ELISA, to ensure optimal conditions for antibody-antigen interactions. Additionally, it is employed in cell culture applications to maintain physiological pH levels, promoting healthy cell growth and function. Its compatibility with various biological samples makes it a versatile choice in laboratory settings.