Gelatin Veronal Buffer with Mg²⁺ and EGTA is primarily used in immunological and biochemical research. It is commonly employed in complement fixation tests, where it helps stabilize and maintain the activity of complement proteins during assays. The buffer's composition, including gelatin, provides a protective environment for proteins, while Mg²⁺ acts as a cofactor for enzymatic reactions. EGTA, a chelating agent, selectively binds calcium ions, making it useful in studies where calcium interference needs to be minimized. This buffer is also utilized in cell culture applications to maintain physiological conditions and support cell viability. Its ability to mimic biological environments makes it a valuable tool in studying immune responses, protein interactions, and cellular processes.