Gelatin Veronal Buffer with EDTA (GVBE) is widely used in immunology and biochemistry for complement fixation tests. It helps stabilize complement components during assays, ensuring accurate detection of antigen-antibody interactions. The buffer is also employed in hemolytic assays to measure complement activity, where it maintains the integrity of red blood cells and complement proteins. Additionally, GVBE is utilized in protein purification processes to prevent metal ion interference, thanks to the chelating properties of EDTA. Its gelatin component provides a protective colloid, enhancing the stability of biological samples during experimental procedures.